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OBJECTIVE:To introduce training mode of American Pharm. D. project,and to provide reference for pharmacy education mode reform and cultivation of new pharmaceutical talents meeting the needs of pharmaceutical care in the new era in China. METHODS:The training mode,teaching concept,course setting and teaching method of American Pharm. D. project were investigated by observing and learning teaching course of pharmacy practice department in University of Nebraska Medical Center (UNMC). The characteristics of American pharmacy education represented by UNMC were summarized,and the suggestion for pharmacy education reform in China was put forward. RESULTS & CONCLUSIONS:Based on the goal of pharmacy talent cultivation for pharmaceutical care,American pharmacy education confirms and improves training process and course system around the career development goal of pharmacists,pays attention to humanistic concern and pharmacist vocational ability training in teaching mode, focuses on modern teaching technology and diversified teaching methods during teaching course, and implements strict formative evaluation to evaluate teaching effectiveness. Chinese pharmacy education should learn from the successful experience of American Pharm. D. project,confirm the training goal of service-oriented pharmaceutical talents,optimize training procedure and course system of clinical pharmacy major adhere to the goal of vocational development,focus on vocational ability and humanistic care training,actively utilize modern educational technology and diversified teaching methods,gradually combin formative evaluation with summative evaluation,and eventually transfer to the formative evaluation-centered evaluation system,so as to cultivate service-oriented pharmaceutical talents who meet the actual needs of China.
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OBJECTIVE:To investigate the possibility of constructing anti-cancer drug in-situ hydrogel with self-assembling peptide RAD16-Ⅰ. METHODS:The rheological parameters as storage modulus(G′),loss modulus(G″)and phase angle(Δ)of 0.1%,0.2% and 0.5% RAD16-Ⅰ solution containing paclitaxel or not were determined by rheometer before and after mixing with isometric phosphate buffer solution (PBS);RAD16-Ⅰ solution containing paclitaxel or not were mixed with breast cancer MDA-MB-435S cells culture medium to obtain hydrogel,the status and effect of which on cell morphology were observed by in-verted microscope (compared with paclitaxel solution). RESULTS:In RAD16-Ⅰ solution containing paclitaxel or not,G′was close to or slightly higher than G″,and G′and G″had changed slightly as the concentration of peptide increased. Compared with not mixed with PBS,G′increased significantly in concentration-dependent manner after mixed with PBS,and G″also increased but was slighter than G′;Δ decreased significantly. In cell culture media,RAD16-Ⅰ solutions containing paclitaxel could form hy-drogel and maintain their gel form,cancer cells kept same morphology after treated with hydrogel and same concentration of pacli-taxel solution for same time. CONCLUSIONS:RAD16-Ⅰ solutions containing paclitaxel can form hydrogel under simulated physi-ological conditions,which can maintain their gel form and have anti-cancer effect of paclitaxel.
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OBJECTIVE:To establish a method for the determination of dissolution of Xiaocaihu pill,and compare the differ-ence of preparation from different manufacturers. METHODS:Using 0.1 mol/L HCl as dissolution medium,rotating basket method was used to determine the dissolution of preparations. HPLC was adopted to determine the content of baicalin:column was TSKgel ODS C18 with mobile phase of methanol-water-phosphoric acid (65∶35∶0.7,V/V/V) at a flow rate of 1 ml/min,detection wave-length was 280 nm,column temperature was 30 ℃,and injection volume was 5 μl. RESULTS:The linear range of baicalin was 0.488-124.8 mg/L (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 100.14%-104.78%(RSD=1.58%,n=9). The average t50(50% dissolution time)of baicalin was 85.81 min. CONCLUSIONS:The method is simple with good precision,stability and reproducibility,and can be used for the dissolution determination of Xiaocaihu pill. Xiaocaihu pill from different manufacturers shows great differences,both preparation formulation and clinical use should attach importance to the dissolution of solid preparations.
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OBJECTIVE: To investigate the feasibility of introducing self-assembling peptides as protein drug carrier.METHODS: By using RAD16-Ⅰ as a model of self-assembling peptides,lysozyme as the model of protein drug,the property of self-assembling peptide solution containing protein to form hydrogel in-situ were characterized by rheology,and the property of protein release in vitro from self-assembling peptide in-situ hydrogel was studied.RESULTS: The self-assembling peptide RAD16-Ⅰ can form hydrogel with certain mechanical intensity rapidly after its solution contained protein being mixed with PBS.Proteins can release easily from RAD16-Ⅰ hydrogel in a sustained-release manner to some extent.About 80% of proteins can be released from the hydrogel within 8 hours and the cumulative protein release from 1.5% hydrogel was less than 90%.The bio-activity of lysozyme released from RAD16-Ⅰ hydrogel was well preserved as 98% to 115%.CONCLUSION: Self-assembling peptide can be employed as potential in-situ hydrogel carrier for protein drugs.
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OBJECTIVE:To establish a RP-HPLC method for determination of Jasminoidin in Yinxie tablets.METHO_ DS:The HPLC system consisted of C 18 column(250mm?4.6mm,5?m),0.01%phosphoric acid-acetonitrile(87∶13)mixture as a mobile phase,detection wavelength at238nm and1.0ml/min of flow rate.RESULTS:The calibration curve of Jasminoidin was linear in the concentration range of20～200?g/ml(r=0.9995),RSD=0.98%(n=5).The average recovery was101.8%(RSD=0.54%).CONCLUSION:This method is simple,quick and specific,and suitable for the quality control of this prepa?ration.
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AIM: To establish quality standard for Yinxie Tablets(Fructus Gardeniae, Radix Astragali, Radix Angelicae Sinensis, etc.). METHODS: Fructus Gardeniae, Radix Astragali and Radix Angelicae Sinensis were identified by TLC, and the content of geniposide was determined by HPLC. RESULTS: Fructus Gardeniae, Radix Astragali and Radix Angelicae Sinensis could be identified by TLC. The calibration curve of geniposide was linear (r=0.999 5). The precision was perfect (RSD=0.98%). The average recovery was 101.81% with RSD of ~0.54% . CONCLUSION: This method is simple, quick and specific, suitable for the quality control.